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The RiboPuromycylation Method (RPM): an Immunofluorescence Technique to Map Translation Sites at the Sub-cellular Level

While isotopic labeling of amino acids remains the reference method in the field for quantifying translation rate, it does not provide any information on spatial localization of translation sites. The rationale behind developing the ribopuromycylation method (RPM) was primarily to map translation si...

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Bibliografische gegevens
Hoofdauteurs: Amandine Bastide, Jonathan Yewdell, Alexandre David
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Bio-protocol LLC 2018-01-01
Reeks:Bio-Protocol
Online toegang:https://bio-protocol.org/en/bpdetail?id=2669&type=0
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