Reporter Gene-Based qRT-PCR Assay for Rho-Dependent Termination In Vivo

In bacteria, the Rho protein mediates Rho-dependent termination (RDT) by identifying a non-specific cytosine-rich Rho utilization site on the newly synthesized RNA. As a result of RDT, downstream RNA transcription is reduced. Due to the bias in reverse transcription and PCR amplification, we could n...

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Hlavní autoři:	Monford Paul Abishek N, Heungjin Jeon, Xun Wang, Heon M. Lim
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	MDPI AG 2023-11-01
Edice:	Cells
Témata:	Rho-dependent termination reporter gene qRT-PCR operon
On-line přístup:	https://www.mdpi.com/2073-4409/12/22/2596
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