Isolation of macrophages from mouse skin wounds for single-cell RNA sequencing

Summary: Understanding macrophage heterogeneity in tissue repair is a major challenge. Here, we describe a protocol that combines isolation of immune cells from skin wounds with subsequent flow-cytometry-based sorting of wound macrophages and single-cell RNA sequencing. We use a modified version of...

Бүрэн тодорхойлолт

Номзүйн дэлгэрэнгүй

Үндсэн зохиолчид:	Sebastian Willenborg, Juliana G. Roscito, Alexander Gerbaulet, Axel Roers, Andreas Dahl, Sabine A. Eming, Susanne Reinhardt
Формат:	Artigo
Хэл сонгох:	Inglês
Хэвлэсэн:	Elsevier 2022-06-01
Цуврал:	STAR Protocols
Нөхцлүүд:	Cell Biology Cell isolation Single Cell Flow Cytometry/Mass Cytometry Sequencing RNAseq
Онлайн хандалт:	http://www.sciencedirect.com/science/article/pii/S2666166722002179
Шошгууд:	Шошго нэмэх Шошго байхгүй, Энэхүү баримтыг шошголох эхний хүн болох!