Design and investigation of CRISPRi tools based on dCasMINI protein
Objective To explore the design of CRISPR interference (CRISPRi) tools based on the deactivated CasMINI (dCasMINI) protein and to evaluate their transcriptional inhibition effects. Methods The tetracycline-on (tet-on) system, flow cytometry, and quantitative reverse transcription polymerase chain re...
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| Autore principale: | |
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| Natura: | Artigo |
| Lingua: | Chinês |
| Pubblicazione: |
Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2024-06-01
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| Serie: | Jichu yixue yu linchuang |
| Soggetti: | |
| Accesso online: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-44-6-821.pdf |
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