Quantifying the global binding and target-search dynamics of epigenetic regulatory factors using live-cell single-molecule tracking

Summary: This protocol provides instructions to track the global dynamics of single epigenetic regulatory factors in live cells. We describe an approach to generate cell lines that stably express HaloTag-fused proteins. We then use live-cell single-molecule tracking to obtain kinetic populations and...

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Detalhes bibliográficos

Principais autores:	Kyle Brown, Samantha Kent, Xiaojun Ren
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Elsevier 2021-12-01
coleção:	STAR Protocols
Assuntos:	Biophysics Single-molecule Assays Cell Biology Cell-based Assays Microscopy Gene Expression
Acesso em linha:	http://www.sciencedirect.com/science/article/pii/S2666166721006651
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