Challenges and insights in the exploration of the low abundance human ocular surface microbiome

PurposeThe low microbial abundance on the ocular surface results in challenges in the characterization of its microbiome. The purpose of this study was to reveal factors introducing bias in the pipeline from sample collection to data analysis of low-abundant microbiomes.MethodsLower conjunctiva and...

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Hlavní autoři:	Elio L. Herzog, Marco Kreuzer, Martin S. Zinkernagel, Denise C. Zysset-Burri
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Frontiers Media S.A. 2023-09-01
Edice:	Frontiers in Cellular and Infection Microbiology
Témata:	low abundance microbiome ocular surface microbiome whole metagenome shotgun sequencing DNA extraction kit nylon flocked swab host DNA depletion
On-line přístup:	https://www.frontiersin.org/articles/10.3389/fcimb.2023.1232147/full
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