Simultaneous amplification of 5′ and 3′ cDNA ends based on template-switching effect and inverse PCR
A new approach for simultaneously amplifying the 5′ and 3′ ends of a desired cDNA is described. The method combines the template-switching effect with inverse PCR, which generates the flanking 5′ and 3′ regions of a certain cDNA with very low or no background. It requires only minimal amounts of tot...
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| Principais autores: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Taylor & Francis Group
2006-02-01
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| coleção: | BioTechniques |
| Acesso em linha: | https://www.future-science.com/doi/10.2144/000112051 |
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