Protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironment

Summary: Pancreatic ductal adenocarcinoma (PDAC) organoids that simulate the tumor microenvironment (TME) are an effective tool to identify how TME affects PDAC malignancy. We present a protocol for generating a fused pancreatic cancer organoid (FPCO) that partly reproduces the TME, including hetero...

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Autores principales:	Kenta Takeuchi, Shunsuke Tabe, Yuya Yamamoto, Kenta Takahashi, Megumi Matsuo, Yasuharu Ueno, Masayuki Ohtsuka, Soichiro Morinaga, Yohei Miyagi, Tomoyuki Yamaguchi, Naoki Tanimizu, Hideki Taniguchi
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	Elsevier 2025-03-01
Colección:	STAR Protocols
Materias:	Cancer Stem Cells Organoids
Acceso en línea:	http://www.sciencedirect.com/science/article/pii/S2666166724007044
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