Construction of the recombinant Listeria monocytogenes mutant expressing green fluorescent protein

A recombinant Listeria monocyogenes mutant expressing green fluorescent protein (GFP) gene was constructed by homologous recombination. The target gene gfp was fused to the actA gene fragment downstream of its promoter and signal sequence. PCR amplification and restriction digestion of the PCR produ...

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Hlavní autoři: KE Chun-lin, SONG Hou-hui, JIANG Ling-li, ZHANG Gui-zhi, FANG Wei-huan
Médium: Artigo
Jazyk:Inglês
Vydáno: Zhejiang University Press 2005-09-01
Edice:浙江大学学报. 农业与生命科学版
Témata:
<italic>Listeria monocytogenes</italic>
homologous recombination
green fluorescent protein
heterologous gene expression
On-line přístup:https://www.academax.com/doi/10.3785/1008-9209.2005.05.0638
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