A HABA dye-based colorimetric assay to detect unoccupied biotin binding sites in an avidin-containing fusion protein

Avidin-biotin binding, the most robust non-covalent protein-ligand interaction occurring in nature, has wide-ranging applications in biotechnology. A frequent challenge in these applications is accurately determining the number of unoccupied biotin binding sites in avidin-containing fusion proteins....

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Autors principals:	Sonia Mukherjee, Pierre Leblanc, Mark C Poznansky, Ann E Sluder
Format:	Artigo
Idioma:	Inglês
Publicat:	Taylor & Francis Group 2024-10-01
Col·lecció:	BioTechniques
Matèries:	avidin binding assay binding site occupancy biotin fusion protein self-assembling vaccine
Accés en línia:	https://www.tandfonline.com/doi/10.1080/07366205.2024.2397288
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