Targeted next-generation sequencing of the 16S-23S rRNA region for culture-independent bacterial identification - increased discrimination of closely related species

Abstract The aim of this study was to develop an easy-to-use culture-free diagnostic method based on next generation sequencing (NGS) of PCR amplification products encompassing whole 16S-23S rRNA region to improve the resolution of bacterial species identification. To determine the resolution of the...

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Autores principales:	Artur J. Sabat, Evert van Zanten, Viktoria Akkerboom, Guido Wisselink, Kees van Slochteren, Richard F. de Boer, Ron Hendrix, Alexander W. Friedrich, John W. A. Rossen, Anna M. D. (Mirjam) Kooistra-Smid
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	Nature Portfolio 2017-06-01
Colección:	Scientific Reports
Acceso en línea:	https://doi.org/10.1038/s41598-017-03458-6
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