Gene Splicing by Overlap Extension: Tailor-Made Genes Using the Polymerase Chain Reaction
Gene Splicing by Overlap Extension or “gene SOEing” is a PCR-based method of recombining DNA sequences without reliance on restriction sites and of directly generating mutated DNA fragments in vitro. By modifying the sequences incorporated into the 5′-ends of the primers, any pair of polymerase chai...
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| Главные авторы: | , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Taylor & Francis Group
2013-03-01
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| Серии: | BioTechniques |
| Online-ссылка: | https://www.future-science.com/doi/10.2144/000114017 |
| Метки: |
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