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Navigating directed evolution efficiently: optimizing selection conditions and selection output analysis

Directed evolution is a powerful tool that can bypass gaps in our understanding of the sequence-function relationship of proteins and still isolate variants with desired activities, properties, and substrate specificities. The rise of directed evolution platforms for polymerase engineering has accel...

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Auteurs principaux: Paola Handal-Marquez, Hoai Nguyen, Vitor B. Pinheiro
Format: Artigo
Langue:Inglês
Publié: Frontiers Media S.A. 2024-10-01
Collection:Frontiers in Molecular Biosciences
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Accès en ligne:https://www.frontiersin.org/articles/10.3389/fmolb.2024.1439259/full
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