Isolation, in vitro culture, and characterization of black crappie, Pomoxis nigromaculatus and white crappie, P. annularis ovarian tissue primary cells

Abstract Investigations were conducted to develop a general protocol for the isolation and in vitro culture of ovary‐derived cells from black crappie, Pomoxis nigromaculatus, and white crappie, P. annularis. Five digestive enzymes: 500 U/mL collagenase type I, 500 U/mL collagenase type IV, 0.05% try...

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Egile Nagusiak:	Sujan Bhattarai, Jacob H. Jones, Nilima N. Renukdas, Anita M. Kelly, Dayan A. Perera
Formatua:	Artigo
Hizkuntza:	Inglês
Argitaratua:	Wiley 2023-12-01
Saila:	Journal of the World Aquaculture Society
Gaiak:	crappie incubation temperature ovarian cell isolation primary cell culture TrypLE™ express
Sarrera elektronikoa:	https://doi.org/10.1111/jwas.12981
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