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Profiling bacterial communities by MinION sequencing of ribosomal operons

Abstract Background An approach utilizing the long-read capability of the Oxford Nanopore MinION to rapidly sequence bacterial ribosomal operons of complex natural communities was developed. Microbial fingerprinting employs domain-specific forward primers (16S rRNA subunit), reverse primers (23S rRN...

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Principais autores: Lee J. Kerkhof, Kevin P. Dillon, Max M. Häggblom, Lora R. McGuinness
Format: Artigo
Sprog:Inglês
Udgivet: BMC 2017-09-01
Serier:Microbiome
Fag:
Microbiota
Ribosomal operon
MinION
Species/strain-level resolution
Online adgang:http://link.springer.com/article/10.1186/s40168-017-0336-9
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