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Protocol for preparing SUV420H1 in complex with the nucleosome containing H2A.Z and H4K20Ecx for structure determination

Summary: The histone lysine methyltransferase SUV420H1 preferentially targets the H2A.Z-containing nucleosome core particle (H2A.Z-NCP) and catalyzes the H4K20me2 modification at replication origins. Here, we present a protocol for preparing SUV420H1 in complex with the nucleosome containing H2A.Z a...

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I tiakina i:
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Ngā kaituhi matua: Li Huang, Zheng Zhou
Hōputu: Artigo
Reo:Inglês
I whakaputaina: Elsevier 2024-09-01
Rangatū:STAR Protocols
Ngā marau:
Urunga tuihono:http://www.sciencedirect.com/science/article/pii/S266616672400460X
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