Standardizing vaginal microbial profiling: evaluating swab materials, storage conditions, and host DNA depletion strategies

Abstract Background Studies on understanding female health from a microbial perspective have proliferated in recent years; however, validated protocols for swab materials, storage conditions, and host DNA depletion remain limited for vaginal microbiome studies. This study investigates these critical...

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Autori principali:	Jiwoo Seo, Ruvie Pearl Araneta, Jung Hwa Lee, Jake Adolf Montecillo, Heon Jong Yoo, Yoo-Young Lee, Chul Min Park, Angela Cho, Hyunsu Lee, Hee Yeun Yoon, Min Ju Kim, Jong Mi Kim, Yoon Hee Lee, Nan Young Lee, Nora Jee-Young Park, Hyung Soo Han, Incheol Seo, Gun Oh Chong
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	BMC 2026-01-01
Serie:	BMC Microbiology
Soggetti:	Vaginal microbiome Swab materials Storage conditions Host DNA depletion 16S rRNA sequencing Shotgun metagenomics
Accesso online:	https://doi.org/10.1186/s12866-025-04523-1
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