Protocol for high-throughput screening of SARS-CoV-2 main protease inhibitors using a robust fluorescence polarization assay

Summary: Discovery of efficacious antiviral agents targeting SARS-CoV-2 main protease (Mpro) is of the highest importance to fight against COVID-19. Here, we describe a simple protocol for high-throughput screening of Mpro inhibitors using a robust fluorescence polarization (FP) assay. Candidate Mpr...

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Autors principals:	Jing Zhang, Haohao Yan, Gangan Yan, Xiaoping Liu, Yanchang Wang, Yunyu Chen
Format:	Artigo
Idioma:	Inglês
Publicat:	Elsevier 2022-12-01
Col·lecció:	STAR Protocols
Matèries:	Biophysics Cell-based assays High throughput screening Protein biochemistry Protein expression and purification
Accés en línia:	http://www.sciencedirect.com/science/article/pii/S2666166722006748
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