A Two-Step PCR Protocol Enabling Flexible Primer Choice and High Sequencing Yield for Illumina MiSeq Meta-Barcoding

High-throughput amplicon sequencing that primarily targets the 16S ribosomal DNA (rDNA) (for bacteria and archaea) and the Internal Transcribed Spacer rDNA (for fungi) have facilitated microbial community discovery across diverse environments. A three-step PCR that utilizes flexible primer choices t...

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Bibliografski detalji

Glavni autori:	Ko-Hsuan Chen, Reid Longley, Gregory Bonito, Hui-Ling Liao
Format:	Artigo
Jezik:	Inglês
Izdano:	MDPI AG 2021-06-01
Serija:	Agronomy
Teme:	MiSeq Illumina ITS 16S rDNA amplicon sequencing metabarcoding
Online pristup:	https://www.mdpi.com/2073-4395/11/7/1274
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