Protocol for the quantitative detection of mtDNA in the supernatants of activated human naive CD8+ T cells
Summary: Here, we present a protocol for the quantitative characterization of human T cell aging. We describe steps for sample collection; peripheral blood mononuclear cell (PBMC) isolation; and the enrichment, assessment, and activation of naive CD8+ T cells. We then detail procedures for supernata...
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| Glavni autori: | , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Elsevier
2025-03-01
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| Serija: | STAR Protocols |
| Teme: | |
| Online pristup: | http://www.sciencedirect.com/science/article/pii/S2666166725000188 |
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