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Generation of Human Mesenchymal Stem Cell 3D Spheroids Using Low-binding Plates

The 3D culture of human mesenchymal stem cells (hMSCs) represents a more physiological environment than classical 2D culture and has been used to enhance the MSC secretome or extend cell survival after transplantation. Here we describe a simple and affordable method to generate 3D spheroids of hMSCs...

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Autori principali: Elena Redondo-Castro, Catriona Cunningham, Jonjo Miller, Stuart Cain, Stuart Allan, Emmanuel Pinteaux
Natura: Artigo
Lingua:Inglês
Pubblicazione: Bio-protocol LLC 2018-08-01
Serie:Bio-Protocol
Accesso online:https://bio-protocol.org/en/bpdetail?id=2968&type=0
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