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Improved dsRNA isolation and purification method validated by viral dsRNA detection using novel primers in Saccharomyces cerevisiae

Accurate genomic sequencing demands high-quality double-stranded RNA (dsRNA). Existing methods for dsRNA extraction from yeast, fungi, and plants primarily rely on cellulose, suitable only for small volume extractions, or the time-consuming lithium chloride precipitation. To streamline the tradition...

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Principais autores: Fernando M.H. Cardoso, Alexandre Elias, Inês Pereira, Isabel Maurício, Olga Matos
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2023-12-01
Serier:MethodsX
Fag:
Viral dsRNA
Phenol extraction
Ammonium sulphate
RT-PCR
Sequencing
Online adgang:http://www.sciencedirect.com/science/article/pii/S2215016123004314
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