Reliable CRISPR/Cas9 Genome Engineering in Caenorhabditis elegans Using a Single Efficient sgRNA and an Easily Recognizable Phenotype
CRISPR/Cas9 genome engineering strategies allow the directed modification of the Caenorhabditis elegans genome to introduce point mutations, generate knock-out mutants, and insert coding sequences for epitope or fluorescent tags. Three practical aspects, however, complicate such experiments. First,...
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| Główni autorzy: | , , , , , , |
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| Format: | Artigo |
| Język: | Inglês |
| Wydane: |
Oxford University Press
2017-05-01
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| Seria: | G3: Genes, Genomes, Genetics |
| Hasła przedmiotowe: | |
| Dostęp online: | http://g3journal.org/lookup/doi/10.1534/g3.117.040824 |
| Etykiety: |
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