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Biomanufacturing Recombinantly Expressed Cripto-1 Protein in Anchorage-Dependent Mammalian Cells Growing in Suspension Bioreactors within a Three-Dimensional Hydrogel Microcarrier

Biotherapeutic soluble proteins that are recombinantly expressed in mammalian cells can pose a challenge when biomanufacturing in three-dimensional (3D) suspension culture systems. Herein, we tested a 3D hydrogel microcarrier for a suspension culture of HEK293 cells overexpressing recombinant Cripto...

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Principais autores: Rachel Lev, Orit Bar-Am, Yoni Lati, Ombretta Guardiola, Gabriella Minchiotti, Dror Seliktar
Formato: Artigo
Idioma:Inglês
Publicado: MDPI AG 2023-03-01
Series:Gels
Assuntos:
hydrogel
recombinant protein
bioreactor
microcarrier
HEK293 cells
protein therapeutics
Acceso en liña:https://www.mdpi.com/2310-2861/9/3/243
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