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Long-read sequencing for fast and robust identification of correct genome-edited alleles: PCR-based and Cas9 capture methods.

<h4>Background</h4>Recent developments in CRISPR/Cas9 genome-editing tools have facilitated the introduction of precise alleles, including genetic intervals spanning several kilobases, directly into the embryo. However, the introduction of donor templates, via homology directed repair, can be errone...

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Principais autores: Christopher V McCabe, Peter D Price, Gemma F Codner, Alasdair J Allan, Adam Caulder, Skevoulla Christou, Jorik Loeffler, Matthew Mackenzie, Elke Malzer, Joffrey Mianné, Krystian J Nowicki, Edward J O'Neill, Fran J Pike, Marie Hutchison, Benoit Petit-Demoulière, Michelle E Stewart, Hilary Gates, Sara Wells, Nicholas D Sanderson, Lydia Teboul
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science (PLoS) 2024-03-01
Serier:PLoS Genetics
Online adgang:https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1011187&type=printable
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