Optimized Lysis-Extraction Method Combined With IS6110-Amplification for Detection of Mycobacterium tuberculosis in Paucibacillary Sputum Specimens

Background: When available, nucleic acid tests (NATs) offer powerful tools to strengthen the potential of tuberculosis (TB) diagnosis assays. The sensitivity of molecular assays is critical for detection of Mycobacterium tuberculosis (MTB) in paucibacillary sputum.Materials and Methods: The impact o...

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Auteurs principaux:	Pratt Kolia-Diafouka, Sylvain Godreuil, Arnaud Bourdin, Severine Carrère-Kremer, Laurent Kremer, Philippe Van de Perre, Edouard Tuaillon
Format:	Artigo
Langue:	Inglês
Publié:	Frontiers Media S.A. 2018-09-01
Collection:	Frontiers in Microbiology
Sujets:	DNA extraction Mycobacterium tuberculosis polymerase chain reaction IS6110 sputum
Accès en ligne:	https://www.frontiersin.org/article/10.3389/fmicb.2018.02224/full
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