Fluorescence Lifetime Multiplexing with Fluorogen-Activating FAST Protein Variants and Red-Shifted Arylidene–Imidazolone Derivative as Fluorogen

Fluorescence-lifetime imaging microscopy (FLIM) is a powerful technique for highly multiplexed imaging in live cells. In this work, we present a genetically encoded FLIM multiplexing platform based on a combination of fluorogen-activating protein FAST and red-shifted fluorogen <b>N871b</b> from the...

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Principais autores:	Aidar R. Gilvanov, Ivan N. Myasnyanko, Sergey A. Goncharuk, Marina V. Goncharuk, Vadim S. Kublitski, Daria V. Bodunova, Svetlana V. Sidorenko, Eugene G. Maksimov, Mikhail S. Baranov, Yulia A. Bogdanova
Formato:	Artigo
Idioma:	Inglês
Publicado em:	MDPI AG 2025-04-01
Colecção:	Biosensors
Assuntos:	arylidene–imidazolone FLIM fluorogen-activating protein
Acesso em linha:	https://www.mdpi.com/2079-6374/15/5/274
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