A rapid and accurate protocol for quantifying living Acanthamoeba castellanii cysts

ABSTRACT The accurate quantification of living Acanthamoeba castellanii (AC) cysts after various interventions is essential for evaluating the effectiveness of AC cyst disinfection and their environmental stability. The current protocol for measuring living AC cysts requires 7 days to complete, posi...

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Autori principali:	Kazushi Matsubara, Ryohei Hirose, Norihide Hasegawa, Takumi Minamiyama, Taku Kano, Akinobu Sai, Minoru Yamada, Takaaki Nakaya
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology 2025-10-01
Serie:	Microbiology Spectrum
Soggetti:	Acanthamoeba castellanii excyst carbon dioxide fetal bovine serum quantification
Accesso online:	https://journals.asm.org/doi/10.1128/spectrum.01170-25
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