A Review of Methodologies for the Detection, Quantitation, and Localization of Free Cysteine in Recombinant Proteins: A Focus on Therapeutic Monoclonal Antibodies

Free-cysteine residues in recombinant biotherapeutics such as monoclonal antibodies can arise from incorrect cellular processing of disulfide bonds during synthesis or by reduction of disulfide bonds during the harvest and purification stage of manufacture. Free cysteines can affect potency, induce...

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Bibliografische Detailangaben

1. Verfasser:	Clive Metcalfe
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Frontiers Media S.A. 2022-06-01
Schriftenreihe:	Frontiers in Molecular Biosciences
Schlagworte:	therapeutic monoclonal antibodies disulfide bond free cysteine quantitative mass spectrometry and post-translation modification
Online-Zugang:	https://www.frontiersin.org/articles/10.3389/fmolb.2022.886417/full
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