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A rapid cloning method employing orthogonal end protection.

We describe a novel in vitro cloning strategy that combines standard tools in molecular biology with a basic protecting group concept to create a versatile framework for the rapid and seamless assembly of modular DNA building blocks into functional open reading frames. Analogous to chemical synthesi...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Arjen J Jakobi, Eric G Huizinga
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Public Library of Science (PLoS) 2012-01-01
Saila:PLoS ONE
Sarrera elektronikoa:http://europepmc.org/articles/PMC3369885?pdf=render
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