Programmable no-nonspecific genetic analytical system via dual-circle-based rolling circle amplification with an efficient CRISPR/Cas12a biosensing strategy

Abstract Ultrasensitive and specific detection of DNA is highly important for early cancer screening and diagnosis. Nucleic acid amplification technology is the most commonly used method for oncogene detection, but nonspecific amplification may occur. We designed a nicking endonuclease (NEase)-media...

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Үндсэн зохиолчид:	Chunfeng Feng, Yunxia Wang, Chang Liu, Feng Dong, Mingjing Guo, Fei Liu, Yan Li, Liqun Zhang
Формат:	Artigo
Хэл сонгох:	Inglês
Хэвлэсэн:	Nature Portfolio 2025-11-01
Цуврал:	Scientific Reports
Нөхцлүүд:	Nicking endonuclease Target recycling No-nonspecific amplification Exponential rolling circle amplification CRISPR/Cas12a
Онлайн хандалт:	https://doi.org/10.1038/s41598-025-24565-9
Шошгууд:	Шошго нэмэх Шошго байхгүй, Энэхүү баримтыг шошголох эхний хүн болох!