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Modeling Site-Specific Nucleotide Biases Affecting Himar1 Transposon Insertion Frequencies in TnSeq Data Sets

ABSTRACT TnSeq is a widely used methodology for determining gene essentiality, conditional fitness, and genetic interactions in bacteria. The Himar1 transposon is restricted to insertions at TA dinucleotides, but otherwise, few site-specific biases have been identified. As a result, most analytical...

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Principais autores: Sanjeevani Choudhery, A. Jacob Brown, Chidiebere Akusobi, Eric J. Rubin, Christopher M. Sassetti, Thomas R. Ioerger
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2021-10-01
coleção:mSystems
Assuntos:
Himar1 transposon
TnSeq
gene essentiality
linear regression models
machine learning
mutant fitness
Acesso em linha:https://journals.asm.org/doi/10.1128/msystems.00876-21
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