Topological barrier to Cas12a activation by circular DNA nanostructures facilitates autocatalysis and transforms DNA/RNA sensing

Abstract Control of CRISPR/Cas12a trans-cleavage is crucial for biosensor development. Here, we show that small circular DNA nanostructures which partially match guide RNA sequences only minimally activate Cas12a ribonucleoproteins. However, linearizing these structures restores activation. Building...

Ausführliche Beschreibung

Bibliografische Detailangaben

Hauptverfasser:	Fei Deng, Yi Li, Biyao Yang, Rui Sang, Wei Deng, Maya Kansara, Frank Lin, Subotheni Thavaneswaran, David M. Thomas, Ewa M. Goldys
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Nature Portfolio 2024-03-01
Schriftenreihe:	Nature Communications
Online-Zugang:	https://doi.org/10.1038/s41467-024-46001-8
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