Easy‐Curing and pH‐Regulated CRISPR‐Cas9 Plasmids for Gene Editing and Plasmid Curing in Lactococcus cremoris

ABSTRACT In this work, we developed a plasmid‐based CRISPR‐Cas9 strategy for editing Lactococcus cremoris, which allows easy generation of plasmid‐free strains with the desired modification. We constructed versatile shuttle vectors based on the theta‐type pAMβ1 promiscuous replicon and p15A ori, exp...

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主要な著者:	Javier Nicolás Garay‐Novillo, José Ángel Ruiz‐Masó, Gloria delSolar, José Luis Barra
フォーマット:	Artigo
言語:	Inglês
出版事項:	Wiley 2024-12-01
シリーズ:	Microbial Biotechnology
主題:	CRISPR‐Cas9 gene‐editing Lactococcus cremoris plasmid curing plasmid stability
オンライン･アクセス:	https://doi.org/10.1111/1751-7915.70060
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