Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors

CRISPR interference (CRISPRi) enables programmable, reversible, and titratable repression of gene expression (knockdown) in mammalian cells. Initial CRISPRi-mediated genetic screens have showcased the potential to address basic questions in cell biology, genetics, and biotechnology, but wider deploy...

Celý popis

Podrobná bibliografie

Hlavní autoři:	Joseph M Replogle, Jessica L Bonnar, Angela N Pogson, Christina R Liem, Nolan K Maier, Yufang Ding, Baylee J Russell, Xingren Wang, Kun Leng, Alina Guna, Thomas M Norman, Ryan A Pak, Daniel M Ramos, Michael E Ward, Luke A Gilbert, Martin Kampmann, Jonathan S Weissman, Marco Jost
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	eLife Sciences Publications Ltd 2022-12-01
Edice:	eLife
Témata:	CRISPR CRISPR interference genetic screen functional genomics knockdown
On-line přístup:	https://elifesciences.org/articles/81856
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!