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Two-Point Fluorescence Detection and Automated Fraction Collection Applied to Constant Denaturant Capillary Electrophoresis

Constant denaturant capillary electrophoresis (CDCE) has been shown to be a sensitive method to detect point mutations in DNA sequences of 100-bp lengths. Here, we report a significant modifications for the instrumental setup that allows a highly accurate prediction of the elution time of DNA fragme...

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Principais autores: P.O. Ekstrøm, R. Wasserkort, M. Minarik, F. Foret, W.G. Thilly
Format: Artigo
Jezik:Inglês
Izdano: Taylor & Francis Group 2000-09-01
Serija:BioTechniques
Online dostop:https://www.future-science.com/doi/10.2144/00293rr01
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