Profiling and verifying the substrates of E3 ubiquitin ligase Rsp5 in yeast cells
Summary: Yeast is an essential model organism for studying protein ubiquitination pathways; however, identifying the direct substrates of E3 in the cell presents a challenge. Here, we present a protocol for using the orthogonal ubiquitin transfer (OUT) cascade to profile the substrate specificity of...
Furkejuvvon:
| Váldodahkkit: | , , , , , , , , , , , , , |
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| Materiálatiipa: | Artigo |
| Giella: | Inglês |
| Almmustuhtton: |
Elsevier
2023-09-01
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| Ráidu: | STAR Protocols |
| Fáttát: | |
| Liŋkkat: | http://www.sciencedirect.com/science/article/pii/S2666166723004562 |
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