Evaluation of Primers Targeting the Diazotroph Functional Gene and Development of NifMAP – A Bioinformatics Pipeline for Analyzing nifH Amplicon Data

Diazotrophic microorganisms introduce biologically available nitrogen (N) to the global N cycle through the activity of the nitrogenase enzyme. The genetically conserved dinitrogenase reductase (nifH) gene is phylogenetically distributed across four clusters (I–IV) and is widely used as a marker gen...

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Bibliografische Detailangaben

Hauptverfasser:	Roey Angel, Maximilian Nepel, Christopher Panhölzl, Hannes Schmidt, Craig W. Herbold, Stephanie A. Eichorst, Dagmar Woebken
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Frontiers Media S.A. 2018-04-01
Schriftenreihe:	Frontiers in Microbiology
Schlagworte:	nitrogen fixation primer evaluation nifH gene Illumina amplicon sequencing NifMAP
Online-Zugang:	http://journal.frontiersin.org/article/10.3389/fmicb.2018.00703/full
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