Simplified Gene-Fragment Phage Display System for Epitope Mapping
We describe a simple and efficient system for epitope mapping by cloning random gene fragments into a specially designed gIIIp-based phage display vector. DNA encoding the antigen of interest is PCR-amplified and partially digested with DNaseI to generate 50–150-bp-long fragments, which are polished...
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| Principais autores: | , , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Taylor & Francis Group
1999-08-01
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| Serier: | BioTechniques |
| Online adgang: | https://www.future-science.com/doi/10.2144/99272st04 |
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