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CTGF knockdown in Vero cells reduces autophagy and adhesion and promotes short-term suspension adaptation

IntroductionVero cells are extensively used in viral vaccine production, but their adaptation to serum-free suspension culture is hindered by excessive autophagy and strong anchorage dependence.MethodsIn this study, we identified Connective Tissue Growth Factor (CTGF/CCN2) as significantly upregulat...

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Principais autores: Runsheng Peng, Renhou Jia, Rong Huang, Muzi Li, Xiaoyun Li, Manlin Zhou, Jiamin Wang, Zilin Qiao, Na Sun
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2026-03-01
coleção:Frontiers in Bioengineering and Biotechnology
Assuntos:
CTGF
Vero cells
autophagy
suspension adaptation
adhesion
virus replication
Acesso em linha:https://www.frontiersin.org/articles/10.3389/fbioe.2026.1777187/full
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