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A modular and optimized single marker system for generating Trypanosoma brucei cell lines expressing T7 RNA polymerase and the tetracycline repressor

Here, we present a simple modular extendable vector system for introducing the T7 RNA polymerase and tetracycline repressor genes into Trypanosoma brucei. This novel system exploits developments in our understanding of gene expression and genome organization to produce a streamlined plasmid optimize...

Ausführliche Beschreibung

Gespeichert in:
Hauptverfasser: S. K. Poon, L. Peacock, W. Gibson, K. Gull, S. Kelly
Format: Artigo
Sprache:Inglês
Veröffentlicht: The Royal Society 2012-01-01
Schriftenreihe:Open Biology
Schlagworte:
trypanosomatid
expression
optimization
inducible
codon
tsetse
Online-Zugang:https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.110037
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