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Isothermal titration calorimetry uncovers substrate promiscuity of bicupin oxalate oxidase from Ceriporiopsis subvermispora

Isothermal titration calorimetry (ITC) may be used to determine the kinetic parameters of enzyme-catalyzed reactions when neither products nor reactants are spectrophotometrically visible and when the reaction products are unknown. We report here the use of the multiple injection method of ITC to ch...

Ausführliche Beschreibung

Gespeichert in:
Hauptverfasser: Hassan Rana, Patricia Moussatche, Lis Souza Rocha, Sofiene Abdellaoui, Shelley D. Minteer, Ellen W. Moomaw
Format: Artigo
Sprache:Inglês
Veröffentlicht: Elsevier 2016-03-01
Schriftenreihe:Biochemistry and Biophysics Reports
Schlagworte:
Oxalate oxidase
Isothermal titration calorimetry
Enzyme kinetics
Cupin
Online-Zugang:http://www.sciencedirect.com/science/article/pii/S240558081630005X
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