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Hairy root transformation system as a tool for CRISPR/Cas9-directed genome editing in oilseed rape (Brassica napus)

Our study examined the mutation efficiency of the CRISPR/Cas9 method for tryptophan aminotransferase BnaTAA1 genes involved in the auxin biosynthesis pathway. We made nine CRISPR/Cas9 constructs with various promoters driving the expression of a Cas9 from Staphylococcus aureus (SaCas9) or a plant-co...

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Principais autores: Veronika Jedličková, Kateřina Mácová, Marie Štefková, Jan Butula, Jana Staveníková, Marek Sedláček, Hélène S. Robert
Formato: Artigo
Idioma:Inglês
Publicado: Frontiers Media S.A. 2022-08-01
Series:Frontiers in Plant Science
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Acceso en liña:https://www.frontiersin.org/articles/10.3389/fpls.2022.919290/full
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