Targeted sequencing of Enterobacterales bacteria using CRISPR-Cas9 enrichment and Oxford Nanopore Technologies

ABSTRACT Sequencing DNA directly from patient samples enables faster pathogen characterization compared to traditional culture-based approaches, but often yields insufficient sequence data for effective downstream analysis. CRISPR-Cas9 enrichment is designed to improve the yield of low abundance seq...

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Autors principals:	Hugh Cottingham, Louise M. Judd, Jessica A. Wisniewski, Ryan R. Wick, Thomas D. Stanton, Ben Vezina, Nenad Macesic, Anton Y. Peleg, Iruka N. Okeke, Kathryn E. Holt, Jane Hawkey
Format:	Artigo
Idioma:	Inglês
Publicat:	American Society for Microbiology 2025-02-01
Col·lecció:	mSystems
Matèries:	Oxford Nanopore CRISPR-Cas9 enrichment Klebsiella Enterobacterales metagenomics
Accés en línia:	https://journals.asm.org/doi/10.1128/msystems.01413-24
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