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Optimized protocol for the generation of an orthotopic colon cancer mouse model and metastasis

Summary: The microenvironment plays an essential role in tumor development and metastatic progression. Here, we describe a simple and rapid protocol to generate tumors in mice using colon cancer cell lines or tumoroids in the correct microenvironment, colonic mucosa. We also detail steps for monitor...

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Principais autores: Sophie Richon, Olivier Zajac, Carlos Perez Gonzalez, Danijela Matic Vignjevic
格式: Artigo
語言:Inglês
出版: Elsevier 2023-03-01
叢編:STAR Protocols
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在線閱讀:http://www.sciencedirect.com/science/article/pii/S2666166722009029
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