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Comparing Raman and NanoSIMS for heavy water labeling of single cells

ABSTRACT Stable isotope probing (SIP) experiments in conjunction with Raman microspectroscopy (Raman) or nano-scale secondary ion mass spectrometry (NanoSIMS) are frequently used to explore single cell metabolic activity in pure cultures as well as complex microbiomes. Despite the increasing popular...

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Principais autores: George A. Schaible, John B. Cliff, Jennifer A. Crandall, Jeremy J. Bougoure, Michael N. Mathuri, Alex L. Sessions, Joseph Atwood, Roland Hatzenpichler
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2025-07-01
Colecção:Microbiology Spectrum
Assuntos:
cell activity
cellular heterogeneity
D2O
heavy water
metabolic activity
NanoSIMS
Acesso em linha:https://journals.asm.org/doi/10.1128/spectrum.01659-24
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