Protocol for formulation and evaluation of phytosterol-based lipid nanoparticles as cholesterol alternatives for mRNA delivery

Summary: We present a protocol for formulating and evaluating lipid nanoparticles (LNPs) that incorporate the plant-derived sterols ginsenoside Rg2 and protopanaxadiol (PPD) as substitutes for cholesterol. The protocol describes mRNA preparation, manual mRNA-LNP encapsulation, and physicochemical ch...

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Detalhes bibliográficos

Principais autores:	Jaewook Lee, Jae Hoon Kim, Seung-Yeul Lee, Jun Hyeong Hong, Tae Jeong Oh, Sungwhan An
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Elsevier 2026-03-01
Colecção:	STAR Protocols
Assuntos:	Model Organisms Biotechnology and bioengineering Chemistry Material sciences
Acesso em linha:	http://www.sciencedirect.com/science/article/pii/S2666166726000456
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