Development of a Counterselectable Transposon To Create Markerless Knockouts from an 18,432-Clone Ordered <named-content content-type="genus-species">Mycobacterium bovis</named-content> Bacillus Calmette-Guérin Mutant Resource

ABSTRACT Mutant resources are essential to improve our understanding of the biology of slow-growing mycobacteria, which include the causative agents of tuberculosis in various species, including humans. The generation of deletion mutants in slow-growing mycobacteria in a gene-by-gene approach in ord...

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Principais autores:	Katlyn Borgers, Kristof Vandewalle, Annelies Van Hecke, Gitte Michielsen, Evelyn Plets, Loes van Schie, Sandrine Vanmarcke, Laurent Schindfessel, Nele Festjens, Nico Callewaert
Formato:	Artigo
Idioma:	Inglês
Publicado:	American Society for Microbiology 2020-08-01
Series:	mSystems
Assuntos:	Mycobacterium tuberculosis complex characterized transposon library optimized Himar1 transposon transposon mutagenesis unmarked mutants
Acceso en liña:	https://journals.asm.org/doi/10.1128/mSystems.00180-20
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