Simultaneous Identification of Functional Antigen-Specific CD8⁺ and CD4⁺ Cells after In Vitro Expansion Using Elongated Peptides

Elongated peptides (EPs), containing possibly one or multiple epitope/s, are increasingly used for the screening of antigen-specific CD8⁺ and CD4⁺ cell responses. Here, we present an in vitro protocol that allows the amplification of antigen-specific cells and the subsequent fu...

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Autori principali:	Juliane Schuhmacher, Leon Kleemann, Jennifer Rebecca Richardson, Elisa Rusch, Hans-Georg Rammensee, Cécile Gouttefangeas
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	MDPI AG 2022-10-01
Serie:	Cells
Soggetti:	T cells immunomonitoring elongated peptides ELISpot intracellular cytokine staining
Accesso online:	https://www.mdpi.com/2073-4409/11/21/3451
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