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Cloning, expression and purification of Pwo polymerase from Pyrococcus woesei

Background and objectives: Pyrococcus woesei is a hyperthermophilic archaea and produces a heat stable polymerase(Pwo polymerase) that has proofreading activity. Materials and Methods: In this study, this microorganism was cultured, its DNA was extracted and the pwo gene polymerase was cloned, expre...

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Bibliografski detalji
Glavni autori: Amir Ghasemi, Ali Hatef Salmanian, Nourkhoda Sadeghifard, Amir-Ahmad Salarian, Mohammad Khalifeh Gholi
Format: Artigo
Jezik:Inglês
Izdano: Tehran University of Medical Sciences 2011-09-01
Serija:Iranian Journal of Microbiology
Teme:
Online pristup:https://ijm.tums.ac.ir/index.php/ijm/article/view/99
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